cathepsin k assay kit (Novus Biologicals)

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Novus Biologicals cathepsin k assay kit

Lysosomal program is upregulated in ATMs independent of Siah2 . A , relative mRNA expression of lysosomal genes in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. B , representative Western blot of lysosomal enzymes relative to β-actin in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. The β-actin panel is the same as used in F . The blot shown in F was reprobed for the CTSK image in B . C , enzyme activity of CTSK and LAL in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. D and E , relative mRNA expression of Atgl ( D ) and Hilpda ( E ) in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. F , representative Western blot of ATGL and HILPDA relative to β-actin in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. G , basal glycerol release in fl/fl and Siah2 MacKO BMDMs and BM-ATMs treated with vehicle or 50 μM Atglistatin or 0.1 μM Lalistat-1 for 24 h. H , glycerol release in fl/fl and Siah2 MacKO BM-ATMs treated with vehicle or 50 μM Atglistatin or 0.1 μM Lalistat-1 for 24 h with or without 0.5 mM IBMX (activated lipolysis). I and J , representative confocal images ( I ) and quantification ( J ) of fl/fl and Siah2 MacKO BM-ATMs stained for BODIPY 493/503 (LD, green ), LysoTracker (lysosomes, magenta ), and DAPI (nuclei, blue ) showing colocalization of LD and lysosomes. The scale bar represents 10 μm. Red signals of LysoTracker were converted to magenta using the LUT function in ImageJ. Statistics are reported as mean ± SD using an unpaired t test with Welch’s correction, and each dot denotes technical replicates representative of three to five independent experiments. A , D , E , G , H , and J , statistics are reported as mean ± SD using two-way ANOVA with Tukey’s multiple comparisons test ( C ). p Values are indicated on the graphs. ATGL, adipose triglyceride lipase; ATM, adipose tissue macrophage; BM-ATM, bone marrow–derived ATM; BMDM, bone marrow–derived macrophage; CTSK, cathepsin K; DAPI, 4′,6-diamidino-2-phenylindole; fl/fl, Siah2 flox/flox ; HILPDA, hypoxia-inducible LD-associated protein; IBMX, 3-isobutyl-1-methylxanthine; LAL, lysosomal acid lipase; LD, lipid droplet; MacKO, Siah2 MacKO ; Siah2, seven in absentia homolog 2.

Cathepsin K Assay Kit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorometric+kit/Cathepsin+K+Assay+Kit+(Fluorometric)/pmc13090517-357-10-14
Average 94 stars, based on 1 article reviews

cathepsin k assay kit - by Bioz Stars, 2026-10

94/100 stars

Images

1) Product Images from "Siah2 regulates lipid uptake in adipose tissue macrophages"

Article Title: Siah2 regulates lipid uptake in adipose tissue macrophages

Journal: The Journal of Biological Chemistry

doi: 10.1016/j.jbc.2026.111380

Figure Legend Snippet: Lysosomal program is upregulated in ATMs independent of Siah2 . A , relative mRNA expression of lysosomal genes in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. B , representative Western blot of lysosomal enzymes relative to β-actin in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. The β-actin panel is the same as used in F . The blot shown in F was reprobed for the CTSK image in B . C , enzyme activity of CTSK and LAL in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. D and E , relative mRNA expression of Atgl ( D ) and Hilpda ( E ) in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. F , representative Western blot of ATGL and HILPDA relative to β-actin in fl/fl and Siah2 MacKO BMDMs and BM-ATMs. G , basal glycerol release in fl/fl and Siah2 MacKO BMDMs and BM-ATMs treated with vehicle or 50 μM Atglistatin or 0.1 μM Lalistat-1 for 24 h. H , glycerol release in fl/fl and Siah2 MacKO BM-ATMs treated with vehicle or 50 μM Atglistatin or 0.1 μM Lalistat-1 for 24 h with or without 0.5 mM IBMX (activated lipolysis). I and J , representative confocal images ( I ) and quantification ( J ) of fl/fl and Siah2 MacKO BM-ATMs stained for BODIPY 493/503 (LD, green ), LysoTracker (lysosomes, magenta ), and DAPI (nuclei, blue ) showing colocalization of LD and lysosomes. The scale bar represents 10 μm. Red signals of LysoTracker were converted to magenta using the LUT function in ImageJ. Statistics are reported as mean ± SD using an unpaired t test with Welch’s correction, and each dot denotes technical replicates representative of three to five independent experiments. A , D , E , G , H , and J , statistics are reported as mean ± SD using two-way ANOVA with Tukey’s multiple comparisons test ( C ). p Values are indicated on the graphs. ATGL, adipose triglyceride lipase; ATM, adipose tissue macrophage; BM-ATM, bone marrow–derived ATM; BMDM, bone marrow–derived macrophage; CTSK, cathepsin K; DAPI, 4′,6-diamidino-2-phenylindole; fl/fl, Siah2 flox/flox ; HILPDA, hypoxia-inducible LD-associated protein; IBMX, 3-isobutyl-1-methylxanthine; LAL, lysosomal acid lipase; LD, lipid droplet; MacKO, Siah2 MacKO ; Siah2, seven in absentia homolog 2.

Techniques Used: Expressing, Western Blot, Activity Assay, Staining, Derivative Assay

Chromatin Immunoprecipitation:

Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat#20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5

DNA Purification:

Imaging:

Conjugation Assay:

Gas Chromatography-Mass Spectrometry:

Polymerase Chain Reaction:

Purification:

Sample Prep:

DNA Sequencing:

RNA Sequencing:

other:

Article Title: Siah2 Regulates Lipid Uptake in Adipose Tissue Macrophages.
Article Snippet: 100 g of protein extracts were pre-cleared with protein A/G magnetic beads (Pierce, Cat# 88802) for 1 hr at 4°C.

Article Title: Imipramine Inhibits Osteosarcoma Invasion via Src Inactivation and Caspase‐Dependent Apoptosis
Article Snippet: Cleaved‐Caspase‐8 , NOVUS Biologicals, USA , NBP2‐54862.

MTT Assay:

Article Title: Cytotoxicity of Bulk-Fill Composites on Stem Cells from Human Exfoliated Deciduous Teeth—An In Vitro Study
Article Snippet: .. The extracts were removed, and 100 μL of MTT solution (0.5 mg/mL in DMEM) was added (MTT Cell Proliferation Assay Kit, Novus Biologicals, Centennial, CO, USA). ..

Proliferation Assay:

Lysis:

Article Title: Brewer's spent grain extract modulates the immune, intercellular junctions and antioxidant gene expression disrupting the Piscirickettsia salmonis infection in rainbow trout intestinal epithelial RTgutGC cells.
Article Snippet: Sustainable mitigation of piscirickettsiosis, the leading infectious cause of mortality in Chilean salmon aquaculture and a key driver of antibiotic dependence, demands green alternatives that fortify the intestinal health of fish.. Here we show that brewer’s spent grain (BSG), an abundant lignocellulosic by-product, can be upgraded into a functional feed additive through a fully aqueous, green-chemistry workflow comprising enzymatic hydrolysis with cellulase/hemicellulase, microwave-vacuum drying, and ultrasound-assisted water extraction.. The process yields a solvent-free extract [bagasse extract–PBS (BEP)1860 μg/mL gallic acid equivalents (GAE)] that preserves ≥90 % RTgutGC cell viability up to 35 μg/mL GAE.

LDH Cytotoxicity Assay:

Activity Assay:

Article Title: Siah2 regulates lipid uptake in adipose tissue macrophages
Article Snippet: Nitrocellulose membranes (Bio-Rad, 0.2 μm) were incubated with primary antibodies for 1 to 2 h at room temperature or overnight at 4°C, followed by 1 h incubation with secondary antibody at room temperature. .. CTSK activity in BMDMs and BM-ATMs was measured using the Cathepsin K assay kit (Novus Biologicals, catalog no.: NBP2-54842) according to the manufacturer’s protocol. ..

Article Title: N6-methyladenosine RNA modification regulates microglial phagocytosis in the APP/PS1 mouse model of Alzheimer's disease.
Article Snippet: .. We assessed the phagocytic activity of microglia by measuring the uptake of PE-labeled Zymosan beads (Novus Biologicals, CO, USA, NBP2-54860). ..

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